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  4. Human 5-hydroxyeicosatetraenoic acid(5-HETE)ELISA Kit

Human 5-hydroxyeicosatetraenoic acid(5-HETE)ELISA Kit

文献引用 (4) 说明书下载
  • 中文名称：
    人5羟二十碳四烯酸(5-HETE)酶联免疫试剂盒
  • 货号：
    CSB-E17006h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 缩写：
    5-HETE
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    7.81 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Biochemicals
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human 5-HETE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 89  
    Range % 84-94  
    1:200 Average % 92  
    Range % 87-99  
    1:400 Average % 93  
    Range % 85-97  
    1:800 Average % 97  
    Range % 90-104  
  • 回收率：
    The recovery of human 5-HETE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 87-97  
    EDTA plasma (n=4) 91 84-96  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    2000 2.739 2.652 2.696 2.586  
    1000 2.258 2.176 2.217 2.107  
    500 1.513 1.504 1.509 1.399  
    250 0.805 0.742 0.774 0.664  
    125 0.434 0.413 0.424 0.314  
    62.5 0.275 0.261 0.268 0.158  
    31.25 0.182 0.179 0.181 0.071  
    0 0.112 0.107 0.110 　  
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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