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  4. Human Cross-linked Carboxy-terminal telopeptide of type Ⅰ collagen, CTX-I ELISA Kit

Human Cross-linked Carboxy-terminal telopeptide of type Ⅰ collagen, CTX-I ELISA Kit

文献引用 (15) 说明书下载
  • 中文名称：
    人Ⅰ型胶原交联羧基末端肽(CTX-I)酶联免疫试剂盒
  • 货号：
    CSB-E11224h
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human CTX-I ELISA Kit was designed for the quantitative measurement of Human CTX-I protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

  • 别名：
    Cross-linked Carboxy-terminal telopeptide of type 1 collagen, CTX-1, Cross-linked Carboxy-terminal telopeptide of type I collagen, CTX-I
  • 缩写：
    CTX-I
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.625 ng/mL-40 ng/mL
  • 灵敏度：
    0.156 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human CTX-I in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 88  
    Range % 83-95  
    1:2 Average % 95  
    Range % 91-98  
    1:4 Average % 97  
    Range % 90-103  
    1:8 Average % 96  
    Range % 91-101  
  • 回收率：
    The recovery of human CTX-I spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 85 81-92  
    EDTA plasma (n=4) 96 93-106  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    40 2.995 2.960 2.978 2.838  
    20 2.557 2.570 2.564 2.424  
    10 1.666 1.765 1.716 1.576  
    5 0.839 0.886 0.863 0.723  
    2.5 0.491 0.524 0.508 0.368  
    1.25 0.263 0.251 0.257 0.117  
    0.625 0.201 0.212 0.207 0.067  
    0 0.138 0.142 0.140 　  
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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■ 常见问题解答
Q:

Please provide the following information regarding Human Cross-linked Carboxy-terminal telopeptide of type I collagen, CTX-I ELISA Kit CSB-E11224h
a. Is the capture antibody a monoclonal or polyclonal?
b. What is the host of the capture antibody?
c. Is the detection antibody a monoclonal or polyclonal?
d. What is the host of the detection antibody?
e. B208. What’s is that standard? A recombinant protein or something else? If a recombinant protein, what is the host?
f. If recombinant protein, is it full length or partial?
g. What is the species of the standard– human or something else?

A:
Thanks for your inquiry.
CSB-E11224h
Capture antibody is mouse monoclonal antibody and detection antibody is goat polyclonal antibody.
Standard is synthetic peptide.
Pls let me know if you have any further questions. Thank you.
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