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  4. Rat dopamine,DA ELISA Kit

Rat dopamine,DA ELISA Kit

文献引用 (41) 说明书下载
  • 中文名称：
    大鼠多巴胺(DA)酶联免疫试剂盒
  • 货号：
    CSB-E08660r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：
        DA (多巴胺，dopamine) 是一种神经递质，在中枢神经系统中发挥重要作用，涉及许多功能，如行为控制、情感调节、内分泌调节和运动协调等。
        华美生物所提供的Rat dopamine,DA ELISA Kit属于ELISA检测试剂盒，采用双抗夹心法定量检测大鼠血清、血浆、组织匀浆样本中的DA，其灵敏度为0.039 ng/mL，检测范围为0.156 ng/mL-10 ng/mL。
     
  • 别名：
    N/A
  • 缩写：
    DA
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat DA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:5 Average % 86  
    Range % 84-96  
    1:10 Average % 95  
    Range % 91-100  
    1:20 Average % 84  
    Range % 81-88  
    1:40 Average % 92  
    Range % 88-96  
  • 回收率：
    The recovery of rat DA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 91 87-95  
    EDTA plasma (n=4) 96 94-98  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    10 2.174 2.280 2.227 2.076  
    5 1.784 1.789 1.787 1.636  
    2.5 1.250 1.276 1.263 1.112  
    1.25 0.841 0.875 0.858 0.707  
    0.625 0.435 0.465 0.450 0.299  
    0.312 0.287 0.308 0.298 0.147  
    0.156 0.208 0.223 0.216 0.065  
    0 0.152 0.150 0.151 　  
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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问答及客户评论

■ 客户评论
  • Sample type: Serum
    Sample species: Rat
    Review: The DA content was evaluated using the corresponding ELISA kit. A similar pattern was observed in the DA striatal content as presented in panel I, where 6-OHDA leveled it off, while the co-administration of 6-OHDA with A-779 depleted it markedly.
    PMID: 29428223
  • Sample type: Cell-free supernatants
    Sample species: Rat
    Review: It was found that 2 hours after the intravenous injection of PC8 a significant increase of melatonin and serotonin (5-HT) was observed in the brain, whereas injection of PITScomposition (PC8+Lip+Xe) decreased the levels of serotonin and dopamine, although there was a considerable rise in the level of melatonin in both cases
    PMID: 30658098
  • Sample type: Supernatants
    Sample species: Rat
    Review: The supernatant was used to measure concentrations of GFAP, DA, TH, MANF, NGF, and CDNF using commercially available ELISA assays following the manufacturer’s instructions. PEx prevents the loss of DA in 6-OHDA challenged rats greater than MIEx (F (5, 29) = 22.03, p< 0.0001). *significant difference between HC and PC. **significant difference between PEx and PC. ***significant difference between PEx and MIEx (P<0.0001).
    PMID: 31100327
  • Sample type: Supernatant of rats’ brains
    Sample species: Rat
    Review: Effects of L-dopa, sitagliptin, or liraglutide alone or combined with L-dopa on striatal dopamine content of rotenone-induced Parkinsonism in rats. L-dopa/carbidopa (50/25 mg/kg, i.p.), sitagliptin (30 mg/kg, p.o.), or liraglutide (50 μg/kg, s.c.) was administered for 16 days alone or in combination to rats treated with 10 daily doses of rotenone (3 mg/kg/day, s.c.)
    PMID: 30673988
  • Sample type: Brain tissue samples
    Sample species: Rat
    Review: Norepinephrine (NE), serotonin (5-HT), and dopamine (DA) levels in the hypothalamus, hippocampus, and prefrontal cortex were measured in brain tissue samples of recommended volume using commercially available enzyme immunoassay kits specifc for each compound as previously described. DA levels (ng/g tissue) in the hippocampus of stress (n=6, outlier number=2) and control ofspring (n=6, outlier number=2). h DA levels (ng/g tissue) in the hypothalamus of stress (n=6, outlier number=2) and control ofspring (n=6, outlier number=2). i DA levels (ng/g tissue) in the prefrontal cortex of stress (n=5, outlier number=3) and control ofspring (n=5, outlier number=3). Control ofspring: ofspring of control group (black); Stress ofspring: ofspring of maternal rats subject to SDS stimulation (grey); 5-HT: serotonin; NE: norepinephrine; DA: dopamine; *p<0.05
    PMID: 30326843
  • Sample type: Supernatant of brain tissues (100 µl)
    Sample species: Rat
    Review: Effect of IDPU treatment on A) Dopamine B) Glutamate C) Calcium D) Nitrite.Values are expressed as mean ± SEM (n = 6). Statistical analysis by one way ANOVA followed by Tukey's post hoc multiple comparison tests. ### P ≤ 0.001, versus sham group, ***P ≤ 0.001 *P ≤ 0.05 indicates versus 6-OHDA (10 µg) lesioned group.
    PMID: 29567422

靶点详情

  • 最新研究进展：
    DA是多巴胺的简称，是一种重要的神经递质。近年来，研究人员发现DA参与了多种生理和病理过程，如运动功能、认知能力、代谢性疾病、精神疾病等。同时，DA与多种神经系统疾病的发病机制密切相关，如帕金森病、精神分裂症等。

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