CD9 Monoclonal Antibody
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货号：CSB-MA004969A0m
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规格：￥2200
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图片：
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Western Blot
Positive WB detected in: U87 whole cell lysate, PC3 whole cell lysate, 293 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, A375 whole cell lysate, MG-63 whole cell lysate
All lanes CD9 antibody at 1:2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 25 KDa
Observed band size: 25 KDa
Exposure time:5min -
Western Blot
Positive WB detected in: A549 whole cell lysate at 20μg, 10μg, 5μg, 2.5μg All lanes: CD9 antibody at 1:2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 25 KDa
Observed band size: 25 KDa
Exposure time:10min -
Western Blot
Positive WB detected in: 20μg A549 whole cell lysate CD9 antibody at 1:2000, 1:4000, 1:8000, 1:16000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 25 KDa
Observed band size: 25 KDa
Exposure time:10min -
Western Blot
Positive WB detected in:
1. Exosomes extracted from plasma
2. Exosomes extracted from serum
3. Exosomes extracted from urine
4. Exosomes extracted in cell supernatant
5. Exosomes extracted from latex
6. Exosomes extracted from saliva
7. Hela whole cell lysate
All lanes CD9 antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 25 KDa
Observed band size: 25 KDa
Exposure time:5min -
Western Blot
Positive WB detected in:
1. Exosomes extracted from saliva
2. Hela whole cell lysate
All lanes Ywhaz antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 25 KDa
Observed band size: 25 KDa
Exposure time:5min -
IHC image of MA004969A0m diluted at 1:50 and staining in paraffin-embedded human breast cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37°C. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB. -
IHC image of MA004969A0m diluted at 1:50 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37°C. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB. -
1. Exosomes extracted from plasma
2. Exosomes extracted from serum
3. Exosomes extracted from urine
4. Exosomes extracted from Hela cells
5. Exosomes extracted from latex
6. Exosomes extracted from saliva
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其他：
产品详情
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产品描述：
This CD9 monoclonal antibody was a laboratory-produced antibody designed to recognize and bind to CD9. Here was the production process. Injected a Recombinant Human CD9 antigen protein into a mouse so that the mouse’s immune response was induced and the B lymphocytes was obtained; Fused the mouse myeloma cells and B lymphocytes to produce hybridoma cells; Selected and cultured the hybridoma cells and finally use them for the production of the CD9 monoclonal antibody, which has been validated in ELISA, WB, IHC.
CD9 protein is a transmembrane glycoprotein that can regulate cell proliferation, movement and differentiation. The protein is transmembrane four times and has a molecular weight of 24KD-27KD. It consists of 227 amino acid residues. Most of them are distributed in the cell membrane, and a few are distributed in the cell plate. Pseudopodia, filopodia and cell vesicles. The protein structure is a single polypeptide chain structure, containing four highly hydrophobic transmembrane domains. The N-terminal and C-terminal are shorter amino acid sequences located in the cytoplasm; the transmembrane domains are separated by hydrophilic groups and are located in the cell The outer hydrophilic group forms two ring structures of varying sizes. The two transmembrane domains and partial amino acid sequences of the ring structure are highly conserved. Some polar amino acids in the transmembrane structure are located in the central part of the ion channel. Participating in the formation of ion channels may be related to intercellular signal transduction. The large loop structure outside the cell contains conservative CCG sequences and cysteine residue sequences, which may be related to the evolution of organisms. Small loops outside the cell The structure contains glycosylation sites. CD9 can be modified by N-glycosylation after transcription. The macrocyclic structure contains monoclonal antibody binding sites. These binding sites are closely related to the function of CD9. -
产品名称：Mouse anti-Homo sapiens (Human) CD9 Monoclonal antibody
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Uniprot No.：
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基因名：CD9
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别名：CD9; MIC3; TSPAN29; GIG2; CD9 antigen; 5H9 antigen; Cell growth-inhibiting gene 2 protein; Leukocyte antigen MIC3; Motility-related protein; MRP-1; Tetraspanin-29; Tspan-29; p24; CD antigen CD9
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宿主：Mouse
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反应种属：Human
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免疫原：Recombinant Human CD9 antigen protein (112-195AA)
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免疫原种属：Homo sapiens (Human)
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标记方式：Non-conjugated
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克隆类型：Monoclonal
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抗体亚型：IgG2a
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纯化方式：>95%, Protein G purified
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克隆号：5A6D8
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浓度：It differs from different batches. Please contact us to confirm it.
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保存缓冲液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 -
产品提供形式：Liquid
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应用范围：ELISA, WB, IHC
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推荐稀释比：
Application Recommended Dilution WB 1:1000-1:16000 IHC 1:50-1:200 -
Protocols：
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储存条件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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货期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
产品评价
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靶点详情
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功能：Integral membrane protein associated with integrins, which regulates different processes, such as sperm-egg fusion, platelet activation and aggregation, and cell adhesion. Present at the cell surface of oocytes and plays a key role in sperm-egg fusion, possibly by organizing multiprotein complexes and the morphology of the membrane required for the fusion. In myoblasts, associates with CD81 and PTGFRN and inhibits myotube fusion during muscle regeneration. In macrophages, associates with CD81 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles. Also prevents the fusion between mononuclear cell progenitors into osteoclasts in charge of bone resorption. Acts as a receptor for PSG17. Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis.
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基因功能参考文献：
- assays. Results from the present study demonstrated that CD9 was highly expressed in the highly metastatic Hepatocellular carcinoma (HCC)cells and promoted HCC cell migration. This protein may be a novel target for regulating the invasive phenotype in HCC. PMID: 29749468
- The species-specific traits in CD9 and CD81 distribution during sperm maturation were compared between mice and humans. A mutual position of CD9/CD81 is shown in human spermatozoa in the acrosomal cap, however in mice, CD9 and CD81 occupy a distinct area. PMID: 29671763
- CD9 expression predicts some clinical characteristics and indicates an unfavorable prognosis in acute lymphoblastic leukemia patients. PMID: 29286918
- CD9-CD81 blockage reduces exosome-mediated HIV-1 entry. PMID: 29429034
- Exosomal markers CD63 and CD9 are elevated in pancreatic tumor tissues. PMID: 28609367
- CD9 expression could be a biomarker for poor prognosis in invasive breast carcinoma PMID: 28178752
- CD9 stabilizes gp130 by blocking its ubiquitin-dependent lysosomal degradation to promote the IL6-gp130-bone marrow X-linked non-receptor tyrosine kinase-STAT3 signaling for maintaining GSC selfrenewal and tumorigenic capacity. PMID: 27740621
- CD9 was highly expressed on extravillous trophoblast (EVT) at the boundary region of EVT invasion and intravascular EVT. CD9 expression on Swan71 cells was reduced under hypoxic conditions, while its expression was increased by co-culture with HUVEC. CD9 could attenuate EVT invasion under the influence of an oxygen environment and maternal endothelial cells, proposing CD9 as a potential regulator of human placentation. PMID: 27780531
- As for 18Lin(-), CD34(-) HSCs are characterized by low expression of the tetraspanin CD9, which promotes homing, and high expression of the peptidase CD26, which inhibits homing. PMID: 28687990
- The findings suggest that, in contrast with previous models, the ligand-binding site of integrin alphaVbeta3, binds to the constant region (helices A and B) of the EC2 domain of CD9, CD81, and CD151 antigens. PMID: 27993971
- Data suggest that CD9 should be further evaluated as a target for glioblastoma treatment. PMID: 26573230
- Collectively, using tetraspanin CD9 in tandem with E-cadherin as a biomarker in renal cell carcinoma will help to not only distinguish between types, but also predict the metastatic potential of RCC. PMID: 26855131
- Data indicate that CD9 is implicated in BCC invasiveness and metastases by cellular mechanisms that involve specific CD9+ plasma membrane protrusions of BCCs. PMID: 25762645
- CD9-enriched microdomains negatively regulate LPS-induced receptor formation by preventing CD14 from accumulating into lipid rafts. [Review] PMID: 26378766
- Results indicate that CD9 downregulation promoted pancreatic cancer cell proliferation and migration through at least in part, enhancing the cell surface expression of EGFR. PMID: 25955689
- CD9 expression is upregulated and its expression is correlated with tumor stage and lymph node metastasis in esophageal squamous cell carcinoma patients PMID: 26045817
- Although the current findings did not prove any hypothesis, the indispensable role of CD9 in fertilization process was not excluded and the precise role of CD9 remains unexplained. [review] PMID: 25536312
- CD9 plays a role in the dysmegakaryopoiesis that occurs in primary myelofibrosis. PMID: 25840601
- High CD9 is associated with B acute lymphoblastic leukemia. PMID: 26320102
- These results suggested that the mechanism underlying CD9-induced suppression of cell proliferation may involve the inhibition of phosphorylation of EGFR and the activity of PI3K/Akt and MAPK/Erk signaling pathways PMID: 25760022
- OY-TES-1 downregulation in liver cancer cells inhibits cell proliferation by upregulating CD and downregulating NANOG. PMID: 25673160
- Low levels of CD9 coincidental with a novel nonsense mutation in glycoprotein Ibbeta in a patient with Bernard-Soulier syndrome. PMID: 26275786
- The cysteine residues involved in the formation of the disulfide bridges in CD9 EC2 were all essential for inhibiting multinucleated giant cell formation but a conserved glycine residue in the tetraspanin-defining 'CCG' motif was not. PMID: 25551757
- alteration in CD9 expression was sufficient to profoundly disrupt cellular actin arrangement and endogenous cell contraction by interfering with RhoA signaling. PMID: 25184334
- The mechanism responsible for this negative regulation exerted by CD9 on LFA-1 adhesion does not involve changes in the affinity state of this integrin but seems to be related to alterations in its state of aggregation. PMID: 26003300
- The results demonstrate that hypoxia regulates CD9 expression and CD9-mediated keratinocyte migration via the p38/MAPK pathway. PMID: 25200404
- Report shows that breast cancer cells contain a nuclear CD9 pool and that the abrogation of CD9 expression results in multipolar mitoses and polynucleation. PMID: 25103498
- this study indicated that sialylation involved in the development of MDR of AML cells probably through ST3GAL5 or ST8SIA4 regulating the activity of PI3K/Akt signaling and the expression of P-gp and MRP1. PMID: 24531716
- switch from alphavbeta5 to alphavbeta6 integrin plays a key role in CD9-regulated cell migration and MMP-9 activation in keratinocytes PMID: 25265322
- High expression of CD9 was statistically associated with older patients PMID: 24553302
- CD9 and CD63 tetraspanins block HIV-1-induced cell-cell fusion at the transition from hemifusion to pore opening. PMID: 24608085
- Loss of CD9 expression is associated with enhancement of invasive potential of malignant mesothelioma. PMID: 24466195
- CD9 and CD151 support integrin-mediated signaling at the immunological synapse. PMID: 24723389
- Introduction of CD9 expression in Raji cells resulted in significantly increased cell proliferation and HDAC activity compared to mock transfected Raji cells. PMID: 24747564
- Heparin-binding epidermal growth factor and CD9 are likely implicated in processes that are highly relevant for MS lesion formation PMID: 24038577
- this study points to EGFR as a key mediator between CD9-mediated pro-MMP-9 release and cellular invasion of HT1080 cells. PMID: 24246676
- The second extracellular loop of CD9 was responsible for the upregulation of MMP-9 production. PMID: 23840773
- This is the first study of the expression and prognostic potential of the tetraspanins in oral dysplasia. PMID: 24201754
- Low CD9 expression is associated with malignant mesothelioma. PMID: 23128478
- Both CD9/CD81-silenced cells and CD151-silenced cells showed delayed alpha3beta1-dependent cell spreading on laminin-332. PMID: 23613949
- Data indicate that CD9 acts as scaffold and assembles a ternary JAM-A-CD9-alphavbeta3 integrin complex from which JAM-A is released upon bFGF stimulation. PMID: 23389628
- these data suggest that CD9 is a novel marker for a human germinal center-B cell subset that is committed to plasma cell lineage. PMID: 23291167
- CD9 overexpression was confirmed in osteotropic cells. CD9 was significantly overexpressed in bone metastases versus primary tumors and visceral metastatic lesions. PMID: 23225418
- tetraspanin CD9 modulates molecular organization of integrins in lymphatic endothelial cells, thereby supporting several functions required for lymphangiogenesis PMID: 23223239
- Low CD9 expression is associated with gallbladder neoplasms PMID: 22613496
- identifies human male germ cells with capability of long-term survival and cell turnover in the xenogeneic testis environment PMID: 22592495
- Knockdown of CD9 by siRNA and blockage of CD9 activity by ALB6 in ovarian cancer cells demonstrated that constitutive activation of NF-kappaB is CD9 dependent and that CD9 is involved in anti-apoptosis PMID: 22095071
- CD9 increases GCM1 expression via the cAMP/PKA signaling pathway, resulting in the increase in ERVWE1 expression. PMID: 19692500
- The absence or down-regulation of CD9 expression and point mutation may play a considerable role in the pathway of the malignant transformation in the BEAS-2B cells induced by mineral powder. PMID: 17997888
- CD9 associates with ADAM17 and, through this interaction, negatively regulates the sheddase activity of ADAM17. PMID: 21365281
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亚细胞定位：Cell membrane; Multi-pass membrane protein. Membrane; Multi-pass membrane protein. Secreted, extracellular exosome.
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蛋白家族：Tetraspanin (TM4SF) family
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组织特异性：Detected in platelets (at protein level). Expressed by a variety of hematopoietic and epithelial cells.
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数据库链接：
